p nitrophenyl phosphate substrate (Thermo Fisher)

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Thermo Fisher p nitrophenyl phosphate substrate
P Nitrophenyl Phosphate Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+nitrophenyl+phosphate/PNPP+Substrate/10__1016_slash_j__aqrep__2026__103811-98-4-7
Average 94 stars, based on 1 article reviews

p nitrophenyl phosphate substrate - by Bioz Stars, 2026-10

94/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Immunostimulatory potential of extracellular vesicles from Limosilactobacillus reuteri EIR/Spx-2, a strain from the gut microbiota of a naturally cancer-resistant host.
Article Snippet: Subsequently, culture supernatants of LrEV- or LPStreated cells and untreated controls were added (50 μL/well), after which the plates were incubated with biotin-labelled antibodies followed by streptavidin–alkaline phosphatase (Mabtech, Sweden). .. Finally, p-nitrophenyl phosphate (Thermo Scientific, USA) was added, and the absorbance was measured at 405 nm using an ELISA reader (BioTek, USA), according to the manufacturer’s instructions. ..

Gene Expression:

Article Title: In-utero cell transplantation for hypophosphatasia with gene-edited hESC-derived MSCs in a murine model.
Article Snippet: TNAP was reacted with p-nitrophenyl phosphate (FUJIFILM, #149–02342) for 30 min, and the p-nitrophenol produced was evaluated using a colorimetric assay at 405 nm (PerkinElmer, 2030 Multilabel Reader). .. One unit was defined as the amount of enzyme that can metabolize 1 μmol of p-nitrophenyl phosphate in 1 min. For analysis of hALPL-D10 gene expression, RNA was extracted from the cells in the dish using ISOGEN (NIPPON GENE, # 311– 02501). cDNA was synthesized using SuperScript IV VILO Master Mix (Invitrogen, #11756050). .. Using the completed cDNA as a template, we quantified the amplified PCR products using Platinum SYBR Green qPCR SuperMix-UDG with ROX (Invitrogen, #11733046) on the Applied Biosystems QuantStudio 12 K Flex Real-Time PCR System.

Synthesized:

other:

Article Title: Live self-destructing bacterial adjuvants to enhance induction of immunity
Article Snippet: Anti-Ova titers or anti-Ag85A, anti-ESAT-6 or anti-CFP-10 titers in the serum (dilution 1:100) were detected with biotinylated goat anti-mouse IgG, IgG1, IgG2a or IgA for Ova and biotinylated goat anti-mouse IgG, IgG1 or IgG2b for Ag85A, ESAT-6 and CFP-10 (Southern Biotechnology) followed by incubation with a streptavidin-alkaline phosphatase conjugate (Southern Biotechnology).

Article Title: Novel approaches methodologies in ecotoxicology - Metabolism reveals multigenerational effects of pollutants on daphnids.
Article Snippet: CaCl2⋅2H2O (CAS 10,035-04–8), MgSO4⋅7H2O (CAS 10,034-99–8), NaHCO3 (CAS 144–55–8), HCl (CAS 7647–01–0), p-nitrophenyl phosphate (CAS 4264–83–9), boric acid (CAS 10,043-35–3), ammonium acetate (CAS 631–61–8), NaOH (CAS 1310–73–2), methanol (CAS 67–56–1), and DMSO (CAS 67–68–5) were purchased from ThermoFisher (Ireland).

Incubation:

Article Title: A Competitive Fluorescence Immunoassay Based on Intermolecular Quenching Using an N-Terminally Fluorescent-Labeled IgG Antibody
Article Snippet: .. After three subsequent washes, alkaline phosphatase-conjugated streptavidin (Promega, Cat. No. V5591, 1:1000 dilution) was added and incubated for 30 min. After three washes, 100 μL of p -nitrophenyl phosphate ( p NPP) solution (Thermo Fisher Scientific, Cat. No. 37621) was added. .. After 10 min incubation at room temperature with shaking, the absorbance at 405 nm was measured using a microplate reader (Multiskan GO, Thermo Fisher Scientific).

Article Title: Biotransformation Is an Effective Mechanism for Modulating the Biological Toxicity of Nodularin (NODR)
Article Snippet: .. Following the mixing process, 90 μL of p -nitrophenyl phosphate ( p -NPP) was added to each well, and the mixture was incubated at room temperature for 1 h. The absorbance at 405 nm (OD 405 ) was quantified utilizing a Thermo/Max microplate reader. ..

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